CyclesSet-class:
Class CyclesSet
Description
Class to contain and describe raw fluorescence data. Extends eSet
Creating Objects
new("CyclesSet")
Slots
assayData
:- Object of class
AssayData
containing the raw data,
which will be a matrix of fluorescence values.
phenoData
:- Object of class
AnnotatedDataFrame
containing phenotypic data for the samples.
annotation
- A character string identifying the
annotation that may be used for the
CyclesSet
instance.
protocolData
:- Object of class
AnnotatedDataFrame
containing protocol data for the samples.
featureData
- Object of class
AnnotatedDataFrame
containing feature-level (e.g., probeset-level) information.
experimentData
:- Object of class "MIAME" containing
experiment-level information.
.__classVersion__
:- Object of class
Versions
describing the R and Biobase version number used to create the
instance. Intended for developer use.
Methods
- exprs
signature(object = "CyclesSet")
: extracts the matrix with
the fluorescence values. - exprs<-
signature(object = "CyclesSet", value = "matrix")
:
replaces the matrix with the fluorescence values.
References
Perkins, JR, Dawes, JM, McMahon, SB, Bennett, DL, Orengo, C, Kohl, M (2012).
ReadqPCR and NormqPCR: R packages for the reading, quality checking and
normalisation of RT-qPCR quantification cycle (Cq) data.
BMC Genomics, 13, 1:296.