InputFrame

InputFrame,data.frame-method

A data.frame with three columns: Sample, Detector, and Ct

object


  Generally an InputFrame is built from a ReaderClass
  (e.g. <code>InputReader</code>), or a data.frame. See the example below
  for building an object from a valid data.frame.


The Delta-Delta-Ct (ddCt) Algorithm is an approximation method to determine relative gene expression with quantitative real-time PCR (qRT-PCR) experiments. Compared to other approaches, it requires no standard curve for each primer-target pair, therefore reducing the working load and yet returning accurate enough results as long as the assumptions of the amplification efficiency hold. The ddCt package implements a pipeline to collect, analyse and visualize qRT-PCR results, for example those from TaqMan SDM software, mainly using the ddCt method. The pipeline can be either invoked by a script in command-line or through the API consisting of S4-Classes, methods and functions.

Jitao David Zhang

ddCt

The ddCt Algorithm for the Analysis of Quantitative Real-Time PCR (qRT-PCR)

InputFrame function

Generally an InputFrame is built from a ReaderClass
  (e.g. <code><linkS4class>InputReader</linkS4class></code>), or a data.frame. See the example below
  for building an object from a valid data.frame.

InputFrame: Build an InputFrame from a ReaderClass or a data frame

Description

Usage

Arguments

Value

Examples