A dataset containing the \(C_T\) values of two genes from a qPCR experiment.
Samples were prepared from human tissues; Brain and kidney (n = 6) each.
Primers for each genes were run in separate reaction tubes.

datasets

Calculates the amplification efficiency and curves from real-time
quantitative PCR (Polymerase Chain Reaction) data. Estimates the relative
expression from PCR data using the double delta CT and the standard curve
methods Livak & Schmittgen (2001) <doi:10.1006/meth.2001.1262>. Tests for
statistical significance using two-group tests and linear regression
Yuan et al. (2006) <doi: 10.1186/1471-2105-7-85>.

Mahmoud Ahmed

Analyzing Real-Time Quantitative PCR Data

ct1 function

A data.frame with 12 rows and 2 variables:<dl class="dl-horizontal">
 <dt>c_myc</dt><dd>\(C_T\) values of the target gene c-myc</dd>
 <dt>GAPDH</dt><dd>\(C_T\) values of the control gene GAPDH</dd>
</dl>

Format

\(C_T\) values from qPCR (separate tubes) — ct1

A data.frame with 12 rows and 2 variables:<dl class='dl-horizontal'>
 <dt>c_myc</dt><dd>\(C_T\) values of the target gene c-myc</dd>
 <dt>GAPDH</dt><dd>\(C_T\) values of the control gene GAPDH</dd>
</dl>

ct1: \(C_T\) values from qPCR (separate tubes)

Description

Usage

Arguments

Format

See Also