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edge (version 2.4.2)

diffSpliceDGE: Test for Differential Exon Usage

Description

Given a negative binomial generalized log-linear model fit at the exon level, test for differential exon usage between experimental conditions.

Usage

diffSpliceDGE(glmfit, coef=ncol(glmfit$design), contrast=NULL, geneid, exonid=NULL,
              prior.count=0.125, verbose=TRUE)

Arguments

glmfit
an DGEGLM fitted model object produced by glmFit or glmQLFit. Rows should correspond to exons.
coef
integer indicating which coefficient of the generalized linear model is to be tested for differential exon usage. Defaults to the last coefficient.
contrast
numeric vector specifying the contrast of the linear model coefficients to be tested for differential exon usage. Length must equal to the number of columns of design. If specified, then takes precedence over coef.
geneid
gene identifiers. Either a vector of length nrow(glmfit) or the name of the column of glmfit$genes containing the gene identifiers. Rows with the same ID are assumed to belong to the same gene.
exonid
exon identifiers. Either a vector of length nrow(glmfit) or the name of the column of glmfit$genes containing the exon identifiers.
prior.count
average prior count to be added to observation to shrink the estimated log-fold-changes towards zero.
verbose
logical, if TRUE some diagnostic information about the number of genes and exons is output.

Value

  • diffSpliceDGE produces an object of class DGELRT containing the component design from glmfit plus the following new components:
  • comparisoncharacter string describing the coefficient being tested.
  • coefficientsnumeric vector of coefficients on the natural log scale. Each coefficient is the difference between the log-fold-change for that exon versus the log-fold-change for the entire gene which contains that exon.
  • genesdata.frame of exon annotation.
  • genecolnamecharacter string giving the name of the column of genes containing gene IDs.
  • exoncolnamecharacter string giving the name of the column of genes containing exon IDs.
  • exon.df.testnumeric vector of testing degrees of freedom for exons.
  • exon.p.valuenumeric vector of p-values for exons.
  • gene.df.testnumeric vector of testing degrees of freedom for genes.
  • gene.p.valuenumeric vector of gene-level testing p-values.
  • gene.Simes.p.valuenumeric vector of Simes' p-values for genes.
  • gene.genesdata.frame of gene annotation.
  • Some components of the output depend on whether glmfit is produced by glmFit or glmQLFit. If glmfit is produced by glmFit, then the following components are returned in the output object:
  • exon.LRnumeric vector of LR-statistics for exons.
  • gene.LRnumeric vector of LR-statistics for gene-level test.
  • If glmfit is produced by glmQLFit, then the following components are returned in the output object:
  • exon.Fnumeric vector of F-statistics for exons.
  • gene.df.priornumeric vector of prior degrees of freedom for genes.
  • gene.df.residualnumeric vector of residual degrees of freedom for genes.
  • gene.Fnumeric vector of F-statistics for gene-level test.
  • The information and testing results for both exons and genes are sorted by geneid and by exonid within gene.

Details

This function tests for differential exon usage for each gene for a given coefficient of the generalized linear model.

Testing for differential exon usage is equivalent to testing whether the exons in each gene have the same log-fold-changes as the other exons in the same gene. At exon-level, the log-fold-change of each exon is compared to the log-fold-change of the entire gene which contains that exon. At gene-level, two different tests are provided. One is converting exon-level p-values to gene-level p-values by the Simes method. The other is using exon-level test statistics to conduct gene-level tests.

Examples

Run this code
# Gene exon annotation
Gene <- paste("Gene", 1:100, sep="")
Gene <- rep(Gene, each=10)
Exon <- paste("Ex", 1:10, sep="")
Gene.Exon <- paste(Gene, Exon, sep=".")
genes <- data.frame(GeneID=Gene, Gene.Exon=Gene.Exon)

group <- factor(rep(1:2, each=3))
design <- model.matrix(~group)
mu <- matrix(100, nrow=1000, ncol=6)
# knock-out the first exon of Gene1 by 90%
mu[1,4:6] <- 10
# generate exon counts
counts <- matrix(rnbinom(6000,mu=mu,size=20),1000,6)

y <- DGEList(counts=counts, lib.size=rep(1e6,6), genes=genes)
gfit <- glmFit(y, design, dispersion=0.05)

ds <- diffSpliceDGE(gfit, geneid="GeneID")
topSpliceDGE(ds)
plotSpliceDGE(ds)

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