limma (version 3.22.7)

normexp.signal: Expected Signal Given Observed Foreground Under Normal+Exp Model

Description

Adjust foreground intensities for observed background using Normal+Exp Model. This function is called by backgroundCorrect and is not normally called directly by the user.

Usage

normexp.signal(par, x)

Arguments

par

numeric vector containing the parameters of the Normal+Exp distribution, see normexp.fit for details.

x

numeric vector of (background corrected) intensities

Value

Numeric vector containing adjusted intensities.

Details

In general the vector normmean is computed conditional on background at each spot.

References

Ritchie, M. E., Silver, J., Oshlack, A., Silver, J., Holmes, M., Diyagama, D., Holloway, A., and Smyth, G. K. (2007). A comparison of background correction methods for two-colour microarrays. Bioinformatics http://bioinformatics.oxfordjournals.org/cgi/content/abstract/btm412

Silver, JD, Ritchie, ME, and Smyth, GK (2009). Microarray background correction: maximum likelihood estimation for the normal-exponential convolution. Biostatistics 10, 352-363. http://biostatistics.oxfordjournals.org/cgi/content/abstract/kxn042

See Also

normexp.fit

An overview of background correction functions is given in 04.Background.