These are the results data from the pds_raw data as calculated by the
Bio-Rad QX100 Droplet Digital PCR System.
A data frame with 64 observations on the following 44 variables.
a factor with levels A01 to H04
a factor with levels Absolute Quantification
a factor with levels ABS
a factor with levels B B + P 10^2
gDNA gDNA + P 10^0 gDNA + P 10^1 gDNA + P 10^-1
gDNA + P 10^2 gDNA + P 10^3 gDNA + P 10^4
a factor with levels Ch1NTC
Ch1Unknown Ch2NTC Ch2Unknown
a factor with levels ileS styA
a factor with levels Manual
a numeric vector
a logical vector
a logical vector
a numeric vector
a numeric vector
a numeric vector
a numeric vector
a numeric vector
a numeric vector
a numeric vector
a numeric vector
a numeric vector
a numeric vector
a logical vector
a logical vector
a logical vector
a logical vector
a logical vector
a logical vector
a logical vector
a numeric vector
a logical vector
a logical vector
a numeric vector
a numeric vector
a numeric vector
a logical vector
a logical vector
a numeric vector
a numeric vector
a numeric vector
a numeric vector
a numeric vector
a numeric vector
a numeric vector
a logical vector
a logical vector
Setup: Duplex assay with constant amount of genomic DNA and six 10-fold dilutions of plasmid DNA with 4 replicates, ranging theoretically from ~ 10^4 to 10^-1 copies/ micro L plus 4 replicates without plasmid DNA. Included are No-gDNA-control and No-template-control, 2 replicates each.
Annotation: FX.Y (X = dilution number, Y = replicate number). Hardware: Bio-Rad QX100 Droplet digital PCR system Details: Genomic DNA isolated from Pseudomonas putida KT2440. Plasmid is pCOM10-StyA::EGFP StyB [Jahn et al., 2013, Curr Opin Biotechnol, Vol. 24 (1): 79-87]. Template DNA was heat treated at 95 degree Celsius for 5 min prior to PCR. Channel 1, primers for genomic DNA marker ileS, Taqman probes (FAM labelled). Channel 2, primers for plasmid DNA marker styA, Taqman probes (HEX labelled).
Jahn et al., 2013, Curr Opin Biotechnol, Vol. 24 (1): 79-87
# NOT RUN {
summary(extract_run(read_QX100(pds), 1L:10))
# }
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