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FEM (version 2.8.0)

GenStatR: Generation of differential expression statistics

Description

Given a gene expression data matrix annotated to entrez gene IDs and a phenotype vector, it will generate statistics of differential expression using limma, at the gene-level, for all pairwise comparisons of phenotype levels.

Usage

GenStatR(exp.m,pheno.v)

Arguments

exp.m
The gene expression data matrix with rownames annotated to entrez gene IDs. If there are multiple rows with the same gene ID, function will average these.
pheno.v
The phenotype vector.

Value

top
A list of matrices, from the limma output, ranking genes according to differential expression between two phenotypes as specified in the contrasts matrix, i.e. top[[i]] will contain the ranking for the i'th contrast.
cont
The contrasts matrix, with columns labeling the contrasts.
avexp
The averaged expression matrix at the gene-level.

Details

References

A systems-level integrative framework for genome-wide DNA methylation and gene expression data identifies differential gene expression modules under epigenetic control. Jiao Y, Widschwendter M, Teschendorff AE. Bioinformatics. 2014;30(16):2360-2366

See Also

Examples

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