GEOquery (version 2.38.4)

Converting: Convert a GDS data structure to a BioConductor data structure

Description

Functions to take a GDS data structure from getGEO and coerce it to limma MALists or ExpressionSets.

Usage

GDS2MA(GDS,do.log2=FALSE,GPL=NULL,AnnotGPL=TRUE,getGPL=TRUE)
GDS2eSet(GDS,do.log2=FALSE,GPL=NULL,AnnotGPL=TRUE,getGPL=TRUE)

Arguments

GDS

The GDS datastructure returned by getGEO

do.log2

Boolean, should the data in the GDS be log2 transformed before inserting into the new data structure

GPL

Either a GPL data structure (from a call to getGEO) or NULL. If NULL, this will cause a call to getGEO to produce a GPL. The gene information from the GPL is then used to construct the genes slot of the resulting limma MAList object or the featureData slot of the ExpressionSet instance.

AnnotGPL

In general, the annotation GPL files will be available for GDS records, so the default is to use these files over the user-submitted GPL files

getGPL

A boolean defaulting to TRUE as to whether or not to download and include GPL information when converting to ExpressionSet or MAList. You may want to set this to FALSE if you know that you are going to annotate your featureData using Bioconductor tools rather than relying on information provided through NCBI GEO. Download times can also be greatly reduced by specifying FALSE.

Value

GDS2MA

A limma MAList

GDS2eSet

An ExpressionSet object

Details

This function just rearranges one data structure into another. For GDS, it also deals appropriately with making the "targets" list item for the limma data structure and the phenoData slot of ExpressionSets.

References

See the limma and ExpressionSet help in the appropriate packages

Examples

## Not run: gds505 <- getGEO('GDS505')
## Not run: MA <- GDS2MA(gds505)
## Not run: eset <- GDS2eSet(gds505)