IntClust (version 0.0.2)

DiffGenes: Differential gene expressions for multiple results

Description

The function DiffGenes will, given the output of a certain method, look for genes that are differentially expressed for each cluster by applying the limma function to that cluster and compare it to all other clusters simultaneously. If a list of outputs of several methods is provided, DiffGenes will perform the limma function for each method.

Usage

DiffGenes(List,Selection=NULL, GeneExpr = NULL, nrclusters = NULL, method = "limma", 
sign = 0.05, topG = NULL, fusionsLog = TRUE, WeightClust = TRUE, 
names = NULL)

Arguments

List

A list of the clustering outputs to be compared. The first element of the list will be used as the reference in ReorderToReference.

Selection

If differential gene expression should be investigated for a specific selection of compounds, this selection can be provided here. Selection can be of the type "character" (names of the compounds) or "numeric" (the number of specific cluster).

GeneExpr

The gene expression matrix or ExpressionSet of the objects. The rows should correspond with the genes.

nrclusters

Optional. The number of clusters to cut the dendrogram in. The number of clusters should not be specified if the interest lies only in a specific selection of compounds which is known by name. Otherwise, it is required.

method

The method to applied to look for DE genes. For now, only the limma method is available

sign

The significance level to be handled.

topG

Overrules sign. The number of top genes to be shown.

fusionsLog

To be handed to ReorderToReference.

WeightClust

To be handed to ReorderToReference.

names

Optional. Names of the methods.

Value

The returned value is a list with an element per method. Each element contains a list per cluster with the following elements:

Compounds

A list with the elements LeadCpds (the compounds of interest) and OrderedCpds (all compounds in the order of the clustering result)

Genes

A list with the elements TopDE (a table with information on the top genes) and AllDE (a table with information on all genes)

Details

The function rearranges the clusters of the methods to a reference method such that a comparison is made easier. Given a list of methods, it calls upon ReorderToReference to rearrange the number of clusters according to the first element of the list which will be used as the reference.

References

SMYTH, G. K. (2004). Linear models and empirical Bayes methods for assessing differential expression in microarray experiments. Statistical Applications in Genetics and Molecular Biology. 3(1).

Examples

# NOT RUN {
data(fingerprintMat)
data(targetMat)
data(geneMat)

MCF7_F = Cluster(fingerprintMat,type="data",distmeasure="tanimoto",normalize=FALSE,
method=NULL,clust="agnes",linkage="ward",gap=FALSE,maxK=55,StopRange=FALSE)
MCF7_T = Cluster(targetMat,type="data",distmeasure="tanimoto",normalize=FALSE,
method=NULL,clust="agnes",linkage="ward",gap=FALSE,maxK=55,StopRange=FALSE)

L=list(MCF7_T ,MCF7_F)

MCF7_FT_DE = DiffGenes(L,GeneExpr=geneMat,nrclusters=7,method="limma",
sign=0.05,topG=10,fusionsLog=TRUE,WeightClust=TRUE)

# }