LncFinder (version 1.1.5)

compute_pI: Compute Theoretical Isoelectric Point

Description

This function is basically a wrapper for function computePI. This function translate DNA sequence into protein, and compute the theoretical isoelectric point (pI) of this protein.

Usage

compute_pI(
  Sequences,
  label = NULL,
  on.ORF = FALSE,
  auto.full = FALSE,
  ambiguous.base = FALSE,
  parallel.cores = 2
)

Value

A dataframe.

Arguments

Sequences

A FASTA file loaded by function read.fasta of seqinr-package.

label

Optional. String. Indicate the label of the sequences such as "NonCoding", "Coding".

on.ORF

Logical. If TRUE, pI will be calculated on the longest ORF region. NOTE: If TRUE, the input has to be DNA sequences. (Default: FALSE)

auto.full

Logical. When on.ORF = TRUE but no ORF can be found, if auto.full = TRUE, pI will be calculated on full sequences automatically; if auto.full is FALSE, the sequences that have no ORF will be discarded. Ignored when on.ORF = FALSE. (Default: FALSE)

ambiguous.base

If TRUE, ambiguous bases are taken into account when translating DNA sequences into proteins.

parallel.cores

Integer. The number of cores for parallel computation. By default the number of cores is 2. Users can set as -1 to run this function with all cores.

Author

HAN Siyu

Details

This function can compute the pI of DNA sequences. Method CPC2 (Kang et al. 2017) uses this feature to identify lncRNAs, and this feature is evaluated in the article LncFinder (Han et al. 2018).

Using this function, the theoretical pI can be computed on full sequence or the longest ORF region. In CPC2, pI is calculated on ORF region.

Examples

if (FALSE) {
data(demo_DNA.seq)
Sequences <- demo_DNA.seq

pI_res <- compute_pI(Sequences, on.ORF = TRUE, auto.full = FALSE, ambiguous.base = FALSE)
}