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The MBmca package provides data sets and lightweight utilities for nucleic acid melting curve analysis and presentation on microbead surfaces.

Installation

MBmca is available on CRAN, so installation is as simple as:

install.packages("MBmca")

You can install the latest development version of the code using the devtools R package.

# Install devtools, if you haven't already.
install.packages("devtools")

library(devtools)
install_github("MBmca", "michbur")

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Install

install.packages('MBmca')

Monthly Downloads

285

Version

1.0.1-3

License

GPL (>= 2)

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Last Published

September 3rd, 2021

Functions in MBmca (1.0.1-3)

diffQ2

Calculation of the melting temperatures (Tm, Tm1D2 and Tm2D2) from the first and the second derivative
MBmca-package

Microbead Surface Nucleic Acid Melting Curve Analysis
DMP

Bimodal melting curve experiment on the surface of microbeads.
MultiMelt

Surface melting curve data from direct hybridization experiment of short oligonucleotides.
mcaSmoother

Function to pre-process melting curve data.
mcaPeaks

Function to estimate the approximate local minima and maxima of melting curve data.
MFIerror

Multiple comparison of the temperature dependent variance of the refMFI
DualHyb

Surface melting curve data from direct hybridization experiment of short oligonucleotide probes with bimodal melting curve pattern.
diffQ

Calculation of the melting temperature (Tm) from the first derivative