MBmca (version 1.0.1-3)

DMP: Bimodal melting curve experiment on the surface of microbeads.

Description

A melting curve experiment with six microbead populations and short oligonucleotide probes (direct hybridization). Detection probes for human VIM (vimentin), MLC-2v (myosin regulatory light chain 2, ventricular/cardiac muscle isoform), SERCA2 (Atp2a2 - ATPase, Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform), HRPT1 (hyperparathyroidism 1) and the artificial sequences Poly(dA)20 (20 nt of dA) and aCS (artificial Control Sequence).

Format

A data frame with the melting curves of six different capture and detection probe pairs on six microbeads populations for VIM, MLC-2v, SERCA2, Poly(dA)20, aCS and HPRT1. First column contains the temperature (in degree Celsius, 1 degree Celsius per step) followed by melting curves of VIM, MLC-2v, SERCA2, Poly(dA)20, aCS and HPRT1 with bimodal melting pattern. The dyes and quencher used were Atto 647N and BHQ2.

T

a numeric vector, Temperature in degree Celsius.

VIM.no.Q

a numeric vector, VIM without quencher and without Poly(dT)20 region.

MLC2v.w.Q.w.dT20

a numeric vector, MLC-2v with quencher-labeled detection probe and fluorescent Poly(dA)20 detection probe.

SERCA2.no.Q.w.dT20

a numeric vector, SERCA2 without quencher-labeled detection probe and Poly(dA)20 detection probe.

PolydA20.w.Q

a numeric vector, Poly(dT)20 with fluorescent Poly(dA)20 detection probe (quencher labeled).

aCS.w.Q.w.dT20

a numeric vector, artificial Control Sequence without quencher-labeled detection probe and fluorescent Poly(dA)20 detection probe.

HPRT1.no.Q.w.dT20

a numeric vector, HPRT1 without quencher-labeled detection probe and fluorescent Poly(dA)20 detection probe.

See Also

MFIerror, mcaSmoother, diffQ, diffQ, MultiMelt, DualHyb

Examples

# NOT RUN {
data(DMP)

# }