Learn R Programming

OlinkAnalyze (version 4.2.0)

olink_lod: Calculate LOD using Negative Controls or Fixed LOD

Description

Calculate LOD using Negative Controls or Fixed LOD

Usage

olink_lod(data, lod_file_path = NULL, lod_method = "NCLOD")

Value

A dataframe with 2 additional columns, LOD and PCNormalizedLOD if lod_method is FixedLOD or NCLOD. When Normalization = "Plate Control", LOD and PCNormalizedLOD are identical.

If lod_method is "Both", 4 additional columns will be added:

  • NCLOD - LOD calculated from negative controls and normalized based on normalization column

  • NCPCNormalizedLOD - PC Normalized LOD calculated from negative controls

  • FixedLOD - LOD calculated from fixed LOD file and normalized based on normalization column

  • FixedPCNormalizedLOD - PC Normalized LOD calculated from fixed LOD file

Arguments

data

npx data file

lod_file_path

location of lod file from Olink. Only needed if lod_method = "FixedLOD" or "Both". Default NULL.

lod_method

method for calculating LOD using either "FixedLOD" or negative controls ("NCLOD"), or both ("Both"). Default NCLOD.