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SCAN.UPC (version 2.14.0)

Single-channel array normalization (SCAN) and Universal exPression Codes (UPC)

Description

SCAN is a microarray normalization method to facilitate personalized-medicine workflows. Rather than processing microarray samples as groups, which can introduce biases and present logistical challenges, SCAN normalizes each sample individually by modeling and removing probe- and array-specific background noise using only data from within each array. SCAN can be applied to one-channel (e.g., Affymetrix) or two-channel (e.g., Agilent) microarrays. The Universal exPression Codes (UPC) method is an extension of SCAN that estimates whether a given gene/transcript is active above background levels in a given sample. The UPC method can be applied to one-channel or two-channel microarrays as well as to RNA-Seq read counts. Because UPC values are represented on the same scale and have an identical interpretation for each platform, they can be used for cross-platform data integration.

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Version

Version

2.14.0

License

MIT

Maintainer

Stephen R Piccolo

Last Published

February 15th, 2017

Functions in SCAN.UPC (2.14.0)

BatchAdjustFromFile

Helper function for applying the ComBat batch-adjusting methodology to an ExpressionSet object
InstallBrainArrayPackage

Helper function for installing BrainArray packages
UPC_TwoColor

Universal exPression Codes (UPC) for two-channel microarrays
UPC_Generic_ExpressionSet

Generic function to apply Universal exPression Codes (UPC) transformation to ExpressionSet objects
SCAN

Single-Channel Array Normalization (SCAN) and Universal exPression Codes (UPC) for Affymetrix microarrays
UPC_RNASeq_Single

Apply Universal exPression Codes (UPC) transformation to a single RNA-Seq sample
UPC_Generic

Generic function to apply Universal exPression Codes (UPC) transformation
ParseMetaFromGtfFile

Helper function to parse length and GC content information from a GTF file.
BatchAdjust

Helper function for applying the ComBat batch-adjusting methodology to an ExpressionSet object
UPC_RNASeq

Universal exPression Codes (UPC) for RNA-Seq data
SCAN_TwoColor

Single-Channel Array Normalization (SCAN) for Agilent two-color expression microarrays