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Seurat (version 2.2.0)

NormalizeData: Normalize Assay Data

Description

Normalize data for a given assay

Usage

NormalizeData(object, assay.type = "RNA",
  normalization.method = "LogNormalize", scale.factor = 10000,
  display.progress = TRUE)

Arguments

object

Seurat object

assay.type

Type of assay to normalize for (default is RNA), but can be changed for multimodal analyses.

normalization.method

Method for normalization. Default is log-normalization (LogNormalize). More methods to be added very shortly.

scale.factor

Sets the scale factor for cell-level normalization

display.progress

display progress bar for scaling procedure.

Value

Returns object after normalization. Normalized data is stored in data or scale.data slot, depending on the method

Examples

Run this code
# NOT RUN {
pbmc_small
pmbc_small <- NormalizeData(object = pbmc_small)

# }

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