The Assay object is the basic unit of Seurat; each Assay stores raw, normalized, and scaled data as well as cluster information, variable features, and any other assay-specific metadata. Assays should contain single cell expression data such as RNA-seq, protein, or imputed expression data.
counts
Unnormalized data such as raw counts or TPMs
data
Normalized expression data
scale.data
Scaled expression data
key
Key for the Assay
var.features
Vector of features exhibiting high variance across single cells
meta.features
Feature-level metadata
misc
Utility slot for storing additional data associated with the assay