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Seurat (version 5.0.3)

LoadXenium: Read and Load 10x Genomics Xenium in-situ data

Description

Read and Load 10x Genomics Xenium in-situ data

Usage

LoadXenium(data.dir, fov = "fov", assay = "Xenium")

ReadXenium( data.dir, outs = c("matrix", "microns"), type = "centroids", mols.qv.threshold = 20 )

Value

LoadXenium: A Seurat object

ReadXenium: A list with some combination of the following values:

  • matrix”: a sparse matrix with expression data; cells are columns and features are rows

  • centroids”: a data frame with cell centroid coordinates in three columns: “x”, “y”, and “cell”

  • pixels”: a data frame with molecule pixel coordinates in three columns: “x”, “y”, and “gene”

Arguments

data.dir

Directory containing all Xenium output files with default filenames

fov

FOV name

assay

Assay name

outs

Types of molecular outputs to read; choose one or more of:

  • “matrix”: the counts matrix

  • “microns”: molecule coordinates

type

Type of cell spatial coordinate matrices to read; choose one or more of:

  • “centroids”: cell centroids in pixel coordinate space

  • “segmentations”: cell segmentations in pixel coordinate space

mols.qv.threshold

Remove transcript molecules with a QV less than this threshold. QV >= 20 is the standard threshold used to construct the cell x gene count matrix.