data("pbmc_small")
pbmc_small1 <- SCTransform(object = pbmc_small, variable.features.n = 20, vst.flavor="v1")
pbmc_small2 <- SCTransform(object = pbmc_small, variable.features.n = 20, vst.flavor="v1")
pbmc_merged <- merge(x = pbmc_small1, y = pbmc_small2)
pbmc_merged <- PrepSCTFindMarkers(object = pbmc_merged)
markers <- FindMarkers(
object = pbmc_merged,
ident.1 = "0",
ident.2 = "1",
assay = "SCT"
)
pbmc_subset <- subset(pbmc_merged, idents = c("0", "1"))
markers_subset <- FindMarkers(
object = pbmc_subset,
ident.1 = "0",
ident.2 = "1",
assay = "SCT",
recorrect_umi = FALSE
)
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