apply_amplification_efficiency

This function applies the <code>est_amplification_efficiency()</code> function to a data.frame object which follows the
standardized format shown in the <code>template_WES_standard_curve</code> data set.

Provides reproducible functions for collating and analyzing
data from environmental sampling studies. Environmental Sampling (ES)
of infectious diseases involves collecting samples from various sources
(such as sewage, water, air, soil, or surfaces) to monitor the presence
of pathogens in the environment. Analysis of ES data often requires the
calculation of Real-Time Quantitative PCR (qPCR) variables, normalizing
ES observations, and analyzing sampling site characteristics. To help
reduce the complexity of these analyses we have implemented tools that
assist with establishing standardized ES data formats, absolute and
relative quantification of qPCR data, estimation of qPCR amplification
efficiency, and collating open-source spatial data for sampling sites.

John R Giles

Tools for Analyzing Wastewater and Environmental Sampling Data

apply_amplification_efficiency function

<dl><dt>standard_curves</dt>
<dd>A data.frame giving the target name, serial diluted concentration of target nucleic acid,
and Ct value from a standard curve assay. Must follow the <code>template_WES_standard_curve</code> standardized format.</dd></dl>

Arguments

Apply PCR amplification efficiency estimation to a data.frame — apply_amplification_efficiency

<dl>

<dt>standard_curves</dt>
<dd>A data.frame giving the target name, serial diluted concentration of target nucleic acid,
and Ct value from a standard curve assay. Must follow the <code>template_WES_standard_curve</code> standardized format.</dd>

</dl>

apply_amplification_efficiency: Apply PCR amplification efficiency estimation to a data.frame

Description

Usage

Value

Arguments

Examples