WGCNA (version 1.25-1)

goodGenesMS: Filter genes with too many missing entries across multiple sets

Description

This function checks data for missing entries and returns a list of genes that have non-zero variance in all sets and pass two criteria on maximum number of missing values in each given set: the fraction of missing values must be below a given threshold and the total number of missing samples must be below a given threshold

Usage

goodGenesMS(multiExpr,
            useSamples = NULL,
            useGenes = NULL,
            minFraction = 1/2,
            minNSamples = ..minNSamples,
            minNGenes = ..minNGenes,
            verbose = 1, indent = 0)

Arguments

multiExpr

expression data in the multi-set format (see checkSets). A vector of lists, one per set. Each set must contain a component data that contains the expression data, with rows corresponding to s

useSamples

optional specifications of which samples to use for the check. Should be a logical vector; samples whose entries are FALSE will be ignored for the missing value counts. Defaults to using all samples.

useGenes

optional specifications of genes for which to perform the check. Should be a logical vector; genes whose entries are FALSE will be ignored. Defaults to using all genes.

minFraction

minimum fraction of non-missing samples for a gene to be considered good.

minNSamples

minimum number of non-missing samples for a gene to be considered good.

minNGenes

minimum number of good genes for the data set to be considered fit for analysis. If the actual number of good genes falls below this threshold, an error will be issued.

verbose

integer level of verbosity. Zero means silent, higher values make the output progressively more and more verbose.

indent

indentation for diagnostic messages. Zero means no indentation, each unit adds two spaces.

Value

• A logical vector with one entry per gene that is TRUE if the gene is considered good and FALSE otherwise. Note that all genes excluded by useGenes are automatically assigned FALSE.

Details

The constants ..minNSamples and ..minNGenes are both set to the value 4. For most data sets, the fraction of missing samples criterion will be much more stringent than the absolute number of missing samples criterion.

See Also

goodGenes, goodSamples, goodSamplesGenes for cleaning individual sets separately;

goodSamplesMS, goodSamplesGenesMS for additional cleaning of multiple data sets together.