Analysis of scale free topology for multiple soft thresholding powers. The aim is to help the user pick an appropriate soft-thresholding power for network construction.
pickSoftThreshold(
data,
dataIsExpr = TRUE,
RsquaredCut = 0.85,
powerVector = c(seq(1, 10, by = 1), seq(12, 20, by = 2)),
removeFirst = FALSE, nBreaks = 10, blockSize = NULL,
corFnc = cor, corOptions = list(use = 'p'),
networkType = "unsigned",
moreNetworkConcepts = FALSE,
gcInterval = NULL,
verbose = 0, indent = 0)pickSoftThreshold.fromSimilarity(
similarity,
RsquaredCut = 0.85,
powerVector = c(seq(1, 10, by = 1), seq(12, 20, by = 2)),
removeFirst = FALSE, nBreaks = 10, blockSize = 1000,
networkType = "unsigned",
moreNetworkConcepts=FALSE,
verbose = 0, indent = 0)
expression data in a matrix or data frame. Rows correspond to samples and columns to genes.
logical: should the data be interpreted as expression (or other numeric) data, or as a similarity matrix of network nodes?
similarity matrix: a symmetric matrix with entries between -1 and 1 and unit diagonal.
desired minimum scale free topology fitting index \(R^2\).
a vector of soft thresholding powers for which the scale free topology fit indices are to be calculated.
should the first bin be removed from the connectivity histogram?
number of bins in connectivity histograms
block size into which the calculation of connectivity should be broken up. If not given,
a suitable value will be calculated using function blockSize and printed if verbose>0. If R runs
into memory problems, decrease this value.
the correlation function to be used in adjacency calculation.
a list giving further options to the correlation function specified in corFnc.
network type. Allowed values are (unique abbreviations of) "unsigned",
"signed", "signed hybrid". See adjacency.
logical: should additional network concepts be calculated? If TRUE, the
function will calculate how the network density, the network heterogeneity, and the network centralization
depend on the power. For the definition of these additional network concepts, see Horvath and Dong (2008).
PloS Comp Biol.
a number specifying in interval (in terms of individual genes) in which garbage
collection will be performed. The actual interval will never be less than blockSize.
integer level of verbosity. Zero means silent, higher values make the output progressively more and more verbose.
indentation for diagnostic messages. Zero means no indentation, each unit adds two spaces.
A list with the following components:
estimate of an appropriate soft-thresholding power: the lowest power for which
the scale free topology fit \(R^2\) exceeds RsquaredCut. If \(R^2\) is below RsquaredCut
for all powers, NA is returned.
a data frame containing the fit indices for scale free topology. The columns
contain the soft-thresholding power, adjusted \(R^2\) for the linear fit, the linear coefficient, adjusted
\(R^2\) for a more complicated fit models, mean connectivity, median connectivity and maximum
connectivity. If input moreNetworkConcepts is TRUE, 3 additional columns containing network
density, centralization, and heterogeneity.
The function calculates weighted networks either by
interpreting data directly as similarity, or first transforming it to similarity of the type
specified by networkType.
The weighted networks are obtained by raising the similarity to the powers given in powerVector.
For each power the scale free topology fit index is calculated
and returned along with other information on connectivity.
On systems with multiple cores or processors,
the function pickSoftThreshold takes advantage of parallel processing if the function
enableWGCNAThreads has been called to allow parallel processing and set up the parallel
calculation back-end.
Bin Zhang and Steve Horvath (2005) "A General Framework for Weighted Gene Co-Expression Network Analysis", Statistical Applications in Genetics and Molecular Biology: Vol. 4: No. 1, Article 17
Horvath S, Dong J (2008) Geometric Interpretation of Gene Coexpression Network Analysis. PLoS Comput Biol 4(8): e1000117