WGCNA (version 1.70-3)

nearestNeighborConnectivityMS: Connectivity to a constant number of nearest neighbors across multiple data sets

Description

Given expression data from several sets and basic network parameters, the function calculates connectivity of each gene to a given number of nearest neighbors in each set.

Usage

nearestNeighborConnectivityMS(multiExpr, nNeighbors = 50, power = 6, 
          type = "unsigned", corFnc = "cor", corOptions = "use = 'p'", 
          blockSize = 1000,
          sampleLinks = NULL, nLinks = 5000, setSeed = 36492,
          verbose = 1, indent = 0)

Arguments

multiExpr

expression data in multi-set format. A vector of lists, one list per set. In each list there must be a component named data whose content is a matrix or dataframe or array of dimension 2 containing the expression data. Rows correspond to samples and columns to genes (probes).

nNeighbors

number of nearest neighbors to use.

power

soft thresholding power for network construction. Should be a number greater than 1.

type

a character string encoding network type. Recognized values are (unique abbreviations of) "unsigned", "signed", and "signed hybrid".

corFnc

character string containing the name of the function to calculate correlation. Suggested functions include "cor" and "bicor".

corOptions

further argument to the correlation function.

blockSize

correlation calculations will be split into square blocks of this size, to prevent running out of memory for large gene sets.

sampleLinks

logical: should network connections be sampled (TRUE) or should all connections be used systematically (FALSE)?

nLinks

number of links to be sampled. Should be set such that nLinks * nNeighbors be several times larger than the number of genes.

setSeed

seed to be used for sampling, for repeatability. If a seed already exists, it is saved before the sampling starts and restored after.

verbose

integer controlling the level of verbosity. 0 means silent.

indent

integer controlling indentation of output. Each unit above 0 adds two spaces.

Value

A matrix in which columns correspond to sets and rows to genes; each entry contains the nearest neighbor connectivity of the corresponding gene.

Details

Connectivity of gene i is the sum of adjacency strengths between gene i and other genes; in this case we take the nNeighbors nodes with the highest connection strength to gene i. The adjacency strengths are calculated by correlating the given expression data using the function supplied in corFNC and transforming them into adjacency according to the given network type and power.

See Also

adjacency, softConnectivity, nearestNeighborConnectivity