a4 (version 1.20.0)

heatmap.expressionSet: Image plot of an expressionSet

Description

Grid version of heatmap function adapted to expressionSet objects with some specific requirements such as the possibility to display subgroups, define colors, adapt text graphical parameters (sizes...). The function also suggests a size appropriate for a device to generate a complete plot with all elements.

Usage

heatmap.expressionSet(eset, col.groups = pData(phenoData(eset))[, "subGroup"], col.orderBy = order(pData(phenoData(eset))[, "subGroup"]), col.groups.sep.width = unit(8, "points"), col.labels = sampleNames(eset), col.labels.sep.width = unit(10, "points"), col.labels.gpar = gpar(cex = 1), col.labels.max.nchar = 20, colors.pergroup = FALSE, colors.groups = NULL, colors.groups.min = rgb(1, 1, 1), colors.max = rgb(1, 0, 0), colors.min = rgb(1, 1, 1), colors.nbreaks = 128, colors.palette = NULL, cell.gpar = gpar(lty = 0), row.groups.sep.height = unit(15, "points"), row.labels.sep.height = unit(10, "points"), row.col.groups.display = ifelse(length(unique(col.groups)) > 1, TRUE, FALSE), row.col.groups.display.height = unit(6, "points"), row.labels.gpar = gpar(cex = 1, col = "black"), row.labels.max.nchar = 45, row.labels = list("SYMBOL", "GENENAME"), row.labels.sep = " - ", row.groups = rep(1, nrow(exprs(eset))), row.order = "none", row.groups.hclust = FALSE, row.groups.hclust.n = 4, distfun = dist, hclustfun = function(d) {
    hclust(d, method = "ward")
}, values.min = 0, values.max = 16, title.gpar = gpar(cex = 1.4), title.main = "This is the title  possibly being very long - it will be splited on several lines or even displayed with dots at the end -- see there (does it work? addendum)", title.just = c("right", "top"), title.maxlines = 4, title.cutpoint = 40, subtitle.gpar = gpar(cex = 1), subtitle.main = "This is subtitle", subtitle.maxlines = 4, subtitle.just = title.just, subtitle.cutpoint = 40, margin.top = unit(2, "lines"), margin.left = unit(2, "lines"), margin.right = unit(2, "lines"), margin.bottom = unit(2, "lines"), legend.display = TRUE, legend.range = "full", legend.data.display = ifelse(legend.range == "full", TRUE, FALSE), legend.gpar = gpar(cex = 1), legend.width = unit(250, "points"), legend.height = unit(40, "points"), ...)

Arguments

eset

expressionSet object

col.groups

Vector specifying sub-groups for individual. Sub-groups are treated separately and can thus on plot have different colors.

col.orderBy

Vector specifying ordering for individual. In case there are sub-groups, individual must first be ordered by sub-groups, but an additional variable gives a way to sort individual within sub-groups.

col.groups.sep.width

Object of class unit (grid package). Width used to visually separate sub-groups of individuals. This can be unit(0,"points") for example for no separation.

col.labels

Character vector for columns labels (individuals), by default taken from phenoData.

col.labels.sep.width

Object of class unit (grid package). Space between image matrix zone and columns labels.

col.labels.gpar

Object of class gpar (grid package). Parameters to be used for labels (cex,...).

col.labels.max.nchar

Integer. Number of maximum characters to be used for labels truncation.

colors.pergroup

Boolean. If TRUE, separate colors are used to color image matrix. Colors defined for groups are used.

colors.groups

Vector. Colors to be used for each group of individual. If NULL (default), colors are taken from column "sampleColor" of expressionSet phenodata.

colors.groups.min

Character vector of length 1 corresponding to a valid color. If colors.groups are provided, a shading if done between color.group and this color (default: white).

colors.max

Character vector of length 1 corresponding to a valid color. See colors details.

colors.min

Character vector of length 1 corresponding to a valid color. See colors details.

colors.nbreaks

Integer. Number of cutpoints used to split the color palette/shading.

colors.palette

Character vector of valid color names.

cell.gpar

Object of class gpar (grid package). Parameters used to format cells, for example to add border (gpar(lty=1)).

row.groups.sep.height

Object of class unit (grid package). Height between rows sub-groups.

row.labels.sep.height

Object of class unit (grid package). Height between image plot zone and rows labels

row.col.groups.display

Boolean. Display or not colored band for subgroups of individuals.

row.col.groups.display.height

Object of class unit (grid package). If row.col.groups.display is TRUE then height used for the displayed band.

row.labels.gpar

Object of class gpar (grid package). Parameters to be used for labels (cex,...).

row.labels.max.nchar

Integer. Number of maximum characters to be used for labels truncation.

row.labels

Character vector or list. If vector, direct labels to be used. If list, elements of the list will be taken from featureData and collapsed using row.labels.sep.

row.labels.sep

In case labels are taken from featureData (list for row.labels), separator used to paste the provided columns.

row.groups

Boolean specifying whether rows are split into sub-groups.

row.order

Either a vector of indices to be used to reorder features (rows) or "none" or "hclust" to use clustering.

row.groups.hclust

Boolean. If row.order equals "hclust", one can ask to split features into sub-groups based on a cut of the clustering dendogram.

row.groups.hclust.n

Integer. If row.order equals "hclust" and row.groups.hclust is TRUE, number of sub-groups.

distfun

Function. For row.order equals "hclust", metric function.

hclustfun

Function. For row.order equals "hclust", clustering function.

values.min

Minimum value for the data range. Values that are inferior are assigned to that value. That ensures a maximal cutpoint for the coloring scale.

values.max

Maximum value for the data range. Values that are superior are assigned to that value. That ensures a maximal cutpoint for the coloring scale.

title.gpar

Object of class gpar (grid package). Parameters to be used for the main title (cex,...).

title.main

Character vector. Main title to be displayed.

title.just

Title justification, one of "center","left","right" (first letter of the word can also be used).

title.maxlines

Maximum number of lines for the title split.

title.cutpoint

Integer. Maximum number of characters a line must have. Title is split into lines according to that cutpoint.

subtitle.gpar

Object of class gpar (grid package). Parameters to be used for the subtitle (cex, col,...).

subtitle.main

Character vector. Subtitle. The subtitle will be split into lines following same rules as used for main title.

subtitle.maxlines

Maximum number of lines for the subtitle split.

subtitle.just

Subtitle justification, one of "center","left","right" (first letter of the word can also be used).

subtitle.cutpoint

Integer. Maximum number of characters a line must have. Subtitle is split into lines according to that cutpoint.

margin.top

Object of class unit (grid package). Top margin.

margin.left

Object of class unit (grid package). Left margin.

margin.right

Object of class unit (grid package). Right margin.

margin.bottom

Object of class unit (grid package). Bottom margin.

legend.display

Boolean. Display or not the legend. Legend is positionned in upper right corner.

legend.range

Character: "full" (default) or "data". If full, color scale legend ranges from values.min to values.max. If "data", range is c(min(data),max(data)).

legend.data.display

Boolean. Display or not color scale legend.

legend.gpar

Object of class gpar (grid package). Parameters to be used for color scale legend axis (cex,...).

legend.width

Object of class unit (grid package). Width for the color scale legend.

legend.height

Object of class unit (grid package). Height for the color scale legend.

...

Additional parameters the function may have. Not used currently.

Value

• The function suggests a size (width, height) for the graphic returned as a vector. A typical usage will be to call the function a first time to get those values and call it again with an output device.

Colors

There are several ways to specify colors used for the image zone. The usual way is to have a shading from colors.groups.min to a color per group (typically the same). By default, a shading is indeed proposed between white (for colors.groups.min) and a same color shared by groups (red for colors.groups.max). The number of possible colors in the shading is determined by colors.nbreaks. In case one asks for distinct colors for groups, only a single value for colors.groups.min is allowed. By default, subgroups colors are taken from phenoData ("sampleColor" column), consequence of colors.groups being NULL. Colors for groups are overided by providing a vector of valid colors for this colors.groups argument. An additional and flexible way to determine colors is to provide a complete palette of possible colors, as a character vector of valid colors (argument colors.palette). Note that in this case the argument colors.nbreaks has no effect as the number of possible values is the length of the palette.

Examples

library(RColorBrewer)
library(dichromat)

library(Biobase)
library(grid)
 pdf.directory=getwd()


load(file.path(getwd(),"expressionSetRma.Rda"))      #expressionSetRma


eset <- expressionSetRma[100:130,pData(phenoData(expressionSetRma))[,"sample"]	##### !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
	exprs(eset)[1,5] <- 13.8
	exprs(eset)[10,7] <- 0.5
	eset2 <- expressionSetRma[200:250,] # ARG
	eset3 <- expressionSetRma[1000:1009,pData(phenoData(expressionSetRma))[,"sample"]	eset4 <- expressionSetRma[100:230,pData(phenoData(expressionSetRma))[,"sample"]	
	eset5 <- expressionSetRma[1:400,] # ARG
	
	# eset <- eset2
	
	pdf(file.path(pdf.directory,"eset.pdf"))
		size <- heatmap.expressionSet(eset,subtitle.main=" ")
	dev.off()
	pdf(file.path(pdf.directory,"eset.pdf"),width=size[1],height=size[2])
		heatmap.expressionSet(eset,subtitle.main=" ")
	dev.off()


pdf(file.path(pdf.directory,"eset2.pdf"))
	size <- heatmap.expressionSet(
			eset2,
			colors.nbreaks = 20,
			colors.pergroup=TRUE,
			legend.range="data",
			row.col.groups.display=FALSE,
			cell.gpar=gpar(lwd=0.5),
			legend.height=unit(50,"points"),
			title.just=c("center","center"),
			title.maxlines=2,
			col.groups.sep.width=unit(0,"points"),
			row.labels=featureNames(eset),
			subtitle.main="This is subtitle",
			row.order="hclust",row.groups.hclust=FALSE,
			title.gpar=gpar(cex=2),
			subtitle.gpar=gpar(cex=1.5)
	)
dev.off()
pdf(file.path(pdf.directory,"eset2.pdf"),width=size[1],height=size[2])
	size <- heatmap.expressionSet(
		eset2,
		colors.nbreaks = 20,
		colors.pergroup=TRUE,
		legend.range="data",
		row.col.groups.display=FALSE,
		cell.gpar=gpar(lwd=0.5),
		legend.height=unit(50,"points"),
		title.just=c("center","center"),
		title.maxlines=2,
		col.groups.sep.width=unit(0,"points"),
		row.labels=featureNames(eset),
		subtitle.main="This is subtitle",
		row.order="hclust",row.groups.hclust=FALSE,
		title.gpar=gpar(cex=2),
		subtitle.gpar=gpar(cex=1.5)

)
dev.off()




	
pdf(file.path(pdf.directory,"eset3.pdf"))
size <- heatmap.expressionSet(
		eset3,
		row.labels.gpar=gpar(cex=0.4,col=c(rep("red",2),rep("black",49))	), # col will correctly be a vector only if no group...
		col.labels.gpar=gpar(cex=0.6),
		colors.nbreaks = 20,
		colors.pergroup=TRUE,
		legend.range="data",
		row.col.groups.display=FALSE,
		cell.gpar=gpar(lwd=0.5),
		legend.height=unit(50,"points"),
		title.just=c("center","center"),
		title.maxlines=2,
		col.groups.sep.width=unit(0,"points"),
		row.labels=featureNames(eset),
		subtitle.main="Essai subtitle",
		row.order="hclust",row.groups.hclust=FALSE,
		interactive=FALSE
)
dev.off()

pdf(file.path(pdf.directory,"eset3.pdf"),width=size[1],height=size[2])
size <- heatmap.expressionSet(
		eset3,
		row.labels.gpar=gpar(cex=0.4,col=c(rep("red",2),rep("black",49))	), # col will correctly be a vector only if no group...
		col.labels.gpar=gpar(cex=0.6),
		colors.nbreaks = 20,
		colors.pergroup=TRUE,
		legend.range="data",
		row.col.groups.display=FALSE,
		cell.gpar=gpar(lwd=0.5),
		legend.height=unit(50,"points"),
		title.just=c("center","center"),
		title.maxlines=2,
		col.groups.sep.width=unit(0,"points"),
		row.labels=featureNames(eset),
		subtitle.main="Essai subtitle",
		row.order="hclust",row.groups.hclust=FALSE,
		interactive=FALSE
)
dev.off()



pdf(file.path(pdf.directory,"eset4.pdf"))
size <- heatmap.expressionSet(
		eset4,
		legend.range="data",
		colors.palette = dichromat(rich.colors(190)[1:128]),
		row.col.groups.display=TRUE,
		title.just=c("left","top"),
		title.maxlines=2,
		row.labels=featureNames(eset),
		subtitle.main="",
		row.order="hclust",row.groups.hclust=FALSE,
)
dev.off()

pdf(file.path(pdf.directory,"eset4.pdf"),width=size[1],height=size[2])
size <- heatmap.expressionSet(
		eset4,
		legend.range="data",
		colors.palette = dichromat(rich.colors(190)[1:128]),
		row.col.groups.display=TRUE,
		title.just=c("left","top"),
		title.maxlines=2,
		row.labels=featureNames(eset),
		subtitle.main="",
		row.order="hclust",row.groups.hclust=FALSE,
)
dev.off()

pdf(file.path(pdf.directory,"eset5.pdf"))
size <- heatmap.expressionSet(eset5,row.order="hclust",row.groups.hclust=FALSE)
dev.off()

pdf(file.path(pdf.directory,"eset5.pdf"),width=size[1],height=size[2])
heatmap.expressionSet(eset5,row.order="hclust",row.groups.hclust=FALSE)
dev.off()