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bioimagetools

Synopsis

bioimagetools is an R package for working with images in 3D and 4D, mostly for biology/microscopy.

  • Read and write TIFF stacks.
  • Functions for filtering and segmentation.
  • Analysis of 3D point patterns.

Installation

The stable version is available on CRAN:

setRepositories(ind=c(1,2))
install.packages("bioimagetools")

The development version is available on github:

setRepositories(ind=c(1,2))
install.packages(c("devtools","tiff","EBImage"))
devtools::install_github("volkerschmid/bioimagetools")

You may need to install additional libraries on your OS before you can install bioimagetools. E.g. on Ubuntu/Debian systems, please execute

sudo apt install libssl-dev libcurl4-openssl-dev libtiff5-dev libfftw3-dev

in the terminal before installing bioimagetools.

Contributors

This package is developed at the BioImaging group at the Department of Statistics, in cooperation with the Biocenter, Department of Biology II, both at LMU Munich.

  • Main development and implementation: Volker J Schmid
  • Biological expertise: Marion and Thomas Cremer, Barbara Hübner, Yolanda Markaki, Jens Popken, Lothar Schermelleh, Daniel Smeets
  • Alpha testing: Priyanka Kukreja, Ramin Norousi and Marius Wagner
  • Contributions by Priyanka Kukreja and Fabian Scheipl

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Version

Install

install.packages('bioimagetools')

Monthly Downloads

300

Version

1.1.8

License

GPL-3

Maintainer

Last Published

May 28th, 2022

Functions in bioimagetools (1.1.8)

crossNN

Compute cross-type nearest neighbor distances
cmoments3d

Computes moments from image objects
bwlabel3d

Binary segmentation in 3d
K.cross.3D

K-function cross-type in 3D
folder.choose

Choose a folder interactively
img

Display an image stack
distance2border

A function to compute the distance from spots to borders of classes
cnnTest

Permutation Test for cross-type nearest neighbor distances
L.cross.3D

L-function cross-type in 3d
nearestClassDistances

Find all distances to next neighbor of all classes
intensity3D

Intensity of a 3d Dataset or a Model
readBMP

Read bitmap files
plotNearestClassDistances

Title Plot nearest class distances
segment

Segmentation of 3D images using EM algorithms
readClassTIF

Read TIF file with classes
readTIF

Read tif stacks
segment.outside

Segmentation of the background of 3D images based on automatic threshold
outside

Segmentation of the background of 3D images based on classes
table.n

Cross Tabulation and Table Creation (including empty classes)
nearestClassDistance

Title Find distance to next neighbour of a specific class
nearest.neighbour.distribution

Nearest neighbor distribution (D curve)
testColoc

Permutation Test for cross-type nearest neighbor distances
filterImage3d

Apply filter to 3D images
writeTIF

Writes image stack into a TIFF file. Wrapper for writeTIFF
spots

Find spots based on threshold and minimum total intensity
standardize

Standardize images
mexican.hat.brush

Mexican hat brush to use with filter2