chipPCR (version 1.0-2)

LAMP: real-time loop-mediated isothermal amplification (LAMP) by Tanner et al. (2012)

Description

Real-time amplification plot of a Loop-mediated isothermal amplification (LAMP) by Tanner et al.(2012) in a CFX96 Real Time System (Bio-Rad Laboratories, Hercules, CA, USA).

Usage

data("LAMP")

Format

A data frame with 115 observations on the following 2 variables.

Time

Time in minutes

Normalized.RFU

RFU (Normalized)

Details

The data were digitized as described by Poisot (2011). The image for data extraction was taken from Figure 4B by Tanner et al.(2012). For further experimental and technical details refer to Tanner et al.(2012).

References

Poisot, T. (2011). The digitize Package: Extracting Numerical Data from Scatterplots. The R Journal 3, 25--26.

Tanner, N.A., Zhang, Y., and Evans, T.C. (2012). Simultaneous multiple target detection in real-time loop-mediated isothermal amplification. BioTechniques 53, 81--89.

Examples

# NOT RUN {
plot(LAMP, main = "LAMP by Tanner et al.(2012)", xlab = "Time (minutes)", 
       ylab = "RFU (Normalized)", col = "red", type = "l")
abline(h = 0.05, lty = 2, col = "grey")
legend("bottomright", c("E. coli (Cy5)"), col = "red", pch = 19)
# }