normalizer

is a vector containing the fluorescence values.

is a argument to use a "none", "minm", "max", "luqn", or
	"zscore" normalization. See Details.

method.norm

is the quantile to be used for the quantile normalization. Ignored if 
<code>method.norm</code> is not equal to "luqn".

<code>normalizer</code> normalizes any data set using a chosen method (see Details). 
It may be used when the data from an experiment have considerable variation regarding the 
background and plateau signal.

 normalization

 manip 

 maximum 

 z-score 

A collection of functions to pre-process amplification curve data from polymerase chain reaction (PCR) or isothermal amplification reactions. Contains functions to normalize and baseline amplification curves, to detect both the start and end of an amplification reaction, several smoothers (e.g., LOWESS, moving average, cubic splines, Savitzky-Golay), a function to detect false positive amplification reactions and a function to determine the amplification efficiency. Quantification point (Cq) methods include the first (FDM) and second approximate derivative maximum (SDM) methods (calculated by a 5-point-stencil) and the cycle threshold method. Data sets of experimental nucleic acid amplification systems ('VideoScan HCU', capillary convective PCR (ccPCR)) and commercial systems are included. Amplification curves were generated by helicase dependent amplification (HDA), ccPCR or PCR. As detection system intercalating dyes (EvaGreen, SYBR Green) and hydrolysis probes (TaqMan) were used. For more information see: Roediger et al. (2015) <doi:10.1093/bioinformatics/btv205>.

normalizer: Normalize data

Description

Usage

Arguments

Value

Details

References

See Also

Examples