chipPCR (version 1.0-2)

refMFI: Class "refMFI"

Description

An S4 class containing the output MFIaggr function. refMFI means referenced Mean Fluorescence Intensity (Roediger et al. 2013)

Value

A class for a matrix of amplifications curve data from a qPCR experiment.

Slots

.Data:

"matrix" containing the "Cycle", "Location" (mean, median), "Deviation" (standard deviation, median absolute deviation), "Coefficient of Variance" (CV, RSD) sequential in the columns.

density:

"density" containing results of the density analysis.

qqnorm.data:

"data.frame" containing data required for Quantile-Quantile plots.

stats:

"numeric" vector containing general statistics.

Methods

qqnorm

signature(y = "refMFI"): plots a normal QQ plot. See qqnorm

qqline

signature(y = "refMFI"): adds a line to QQ plot. See qqline

plot

signature(x = "refMFI"): plots the object. See plot.refMFI

show

signature(object = "refMFI"): prints only .Data slot of the object.

summary

signature(object = "refMFI"): prints general statistics and allows easy access to stats slot. See summary.refMFI

References

Roediger S, Boehm A, Schimke I. Surface Melting Curve Analysis with R. The R Journal 2013;5:37--53.

See Also

MFIaggr, plot.refMFI, summary.refMFI

Examples

# NOT RUN {
data(VIMCFX96_60)

res <- MFIaggr(VIMCFX96_60)
#summarize analysis
summary(res)
#plot results of the analysis
plot(res)
#plot a QQ plot
qqnorm(res)
#add QQ line
qqline(res)
# }