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ddpcr (version 1.15.2)

calculate_concentration: Calculate template concentration

Description

Calculate template concentration in each wells in a plate using the same formula that QuantaSoft uses. The concentration information is added to the plate's metadata.

Usage

calculate_concentration(plate)

Arguments

Value

A ddPCR plate with the metadata containing a new `concentration` variable.

Details

The concentration in a well as number of copies of template per microlitre of sample, and uses the following equation:

(-log(drops_empty / drops_total) * drops_total) / (droplet_volume * drops_total)