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ddpcr (version 1.15.2)

classify_droplets: Analysis step: Classify droplets

Description

The main analysis step for ddPCR plates of type pnpp_experiment. Classify each droplet as either rain, ++, or +-. Also calculate the frequency of negative droplets, and attempt to detemine if each well has a statistically significant number of such droplets.

See the README for more information about the algorithm used.

Usage

classify_droplets(plate)

Value

A ddPCR plate with all droplets assigned to a cluster. The plate's metadata will have several new variables.

Arguments

plate

A ddPCR plate.

Details

This function is recommended to be run as part of an analysis pipeline (ie. within the analyze function) rather than being called directly.

See Also

analyze
classify_droplets_single
mark_clusters
has_signif_negative_cluster