The main analysis step for ddPCR plates of type pnpp_experiment
.
Classify each droplet as either rain, ++, or +-. Also calculate the frequency
of negative droplets, and attempt to detemine if each well has a statistically
significant number of such droplets.
See the README for
more information about the algorithm used.
classify_droplets(plate)
A ddPCR plate with all droplets assigned to a cluster. The plate's metadata will have several new variables.
A ddPCR plate.
This function is recommended to be run as part of an analysis pipeline (ie.
within the analyze
function) rather than being called
directly.
analyze
classify_droplets_single
mark_clusters
has_signif_negative_cluster