ddpcr (version 1.15.2)

get_filled_drops: Get filled droplets in PNPP experiment

Description

In a PNPP experiment, the rain droplets are the non-empty drops that don't have a high enough intensity in the positive dimension to be considered as filled with high quality sample DNA. Only droplets considered as filled are candidates for the negative and positive clusters. get_filled_drops returns the droplets that are considered filled.

Usage

get_filled_drops(plate, well_id, border)

Value

Dataframe with all filled droplets in the given well.

Arguments

plate

A ddPCR plate.

well_id

Get border of filled droplets for this well.

border

(Optional) The filled droplets border, as calculated by get_filled_drops. If missing, then get_filled_drops is called to calculate the border.

See Also

pnpp_experiment
positive_dim
get_filled_drops

Examples

file <- system.file("sample_data", "small", "analyzed_pnpp.rds", package = "ddpcr")
plate <- load_plate(file)
get_filled_drops(plate, "A05")
get_filled_drops(plate, "A05", get_filled_border(plate, "A05"))