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dpcR (version 0.5)

ddpcRquant: Quantify droplets

Description

Cluster raw data from QX100 and QX200 systems.

Usage

ddpcRquant(path, threshold.int = 0.9995, reps = 10, blocks = 150,
  threshold.manual = NULL)

Arguments

path

character path to diectory with raw data.

threshold.int

numeric probability of the threshold quantile.

reps

numeric vector representing the number of replications.

blocks

numeric vector representing the number of blocks.

threshold.manual

if numeric, the value is used as the threshold. If NULL, the threshold is calculated automatically.

Value

'>dpcr object.

References

Trypsteen W, Vynck M, De Neve J, Bonczkowski P, Kiselinova M, Malatinkova E, Vervisch K, Thas O, Vandekerckhove L, De Spiegelaere W, ddpcRquant: Threshold Determination for Single Channel Droplet Digital PCR Experiments. Analytical and Bioanalytical Chemistry 2015. 407(19): p.5827-34.