Subset of raw data from the pds_raw data set as measured by the
Bio-Rad QX100 Droplet Digital PCR System.
A list of 3 data frames.
| ExptType
| Absolute Quantification
| Absolute Quantification
| Absolute Quantification
The results can be calculated as by the Bio-Rad QX100 Droplet Digital PCR
System are to be found in pds.
The results can be calculated as by the Bio-Rad QX100 Droplet Digital PCR
System are to be found in pds.
Setup: Duplex assay with a constant amount of genomic DNA and six 10-fold dilutions of plasmid DNA with 4 replicates, ranging theoretically from ~ 10^4 to 10^-1 copies/ micro L plus 4 replicates without plasmid DNA. Included are No-gDNA-control and No-template-control, 2 replicates each.
Annotation: FX.Y (X = dilution number, Y = replicate number). Hardware: Bio-Rad QX100 Droplet digital PCR system Details: Genomic DNA isolated from Pseudomonas putida KT2440. Plasmid is pCOM10-StyA::EGFP StyB [Jahn et al., 2013, Curr Opin Biotechnol, Vol. 24 (1): 79-87]. Template DNA was heat treated at 95 degree Celsius for 5 min prior to PCR. Channel 1, primers for genomic DNA marker ileS, Taqman probes (FAM labelled). Channel 2, primers for plasmid DNA marker styA, Taqman probes (HEX labelled).
#' Setup: Duplex assay with a constant amount of genomic DNA and six 10-fold dilutions of plasmid DNA with 4 replicates, ranging theoretically from ~ 10^4 to 10^-1 copies/ micro L plus 4 replicates without plasmid DNA. Included are No-gDNA-control and No-template-control, 2 replicates each.
Annotation: FX.Y (X = dilution number, Y = replicate number). Hardware: Bio-Rad QX100 Droplet digital PCR system Details: Genomic DNA isolated from Pseudomonas putida KT2440. Plasmid is pCOM10-StyA::EGFP StyB [Jahn et al., 2013, Curr Opin Biotechnol, Vol. 24 (1): 79-87]. Template DNA was heat treated at 95 degree Celsius for 5 min prior to PCR. Channel 1, primers for genomic DNA marker ileS, Taqman probes (FAM labelled). Channel 2, primers for plasmid DNA marker styA, Taqman probes (HEX labelled).
The full data are located at https://github.com/michbur/dpcR_data as QX100_rawdata.zip.
Jahn et al., 2013, Curr Opin Biotechnol, Vol. 24 (1): 79-87
Jahn M, Vorpahl C, Tuerkowsky D, Lindmeyer M, Buehler B, Harms H, et al. Accurate Determination of Plasmid Copy Number of Flow-Sorted Cells using Droplet Digital PCR. Anal Chem 2014; 86:5969--76. doi:10.1021/ac501118v.
# NOT RUN {
#str(pds_raw)
bioamp(data = pds_raw[["D02"]], main = "Well D02", pch = 19)
# }
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