factDesign (version 1.48.0)

estrogen: Microarray Data from an Experiment on Breast Cancer Cells

Description

Gene expression levels for 500 genes from a 2x2 factorial experiment on MCF7 breast cancer cells using Affymetrix HGU95av2 arrays.

Usage

data(estrogen)

Format

An ExpressionSet object with 500 genes, 8 samples, and 2 variables.

Source

Scholtens et al. Analyzing Factorial Designed Microarray Experiments. Journal of Multivariate Analysis. 2004;90(1):19-43.

Details

The factors in this experiment were estrogen (ES: P or A) and length of exposure (TIME: 10 or 48 hours). Gene expression values were estimated using rma after quantile normalization (see the 'affy' package). Expression estimates are reported log base 2, as suggested by the rma method.

Examples

data(estrogen)
pData(estrogen)
exprs(estrogen)[1,]