fcros (version 1.6.1)

voomReads: Transformation of read count values

Description

This function allows to transform the count values associated with Sequencing reads. The purpose of this transformation is to allow applying normal-based microarray-like statistical methods to RNA-seq read counts. Log2 values are returned.

Usage

voomReads(x, Rm=1e+06)

Arguments

x

This is a read counts matrix

Rm

A constant used in the transformation

Value

This function returns a data matrix of the same size as input matrix x. The values of this matrix are expressed in log2 scale.

References

Charity W Law, Yunshun Chen, Wei Shi and Gordon K Smyth, voom: precision weights unlock linear model analysis tools for RNA-seq read counts,Genome Biology, 2014, 15R29

Examples

# NOT RUN {
#   data(fdata);
# }