Dithionite Scramblase Assay Analysis
Description
The lipid scrambling activity of protein extracts and purified
scramblases is often determined using a fluorescence-based assay involving
many manual steps. flippant offers an integrated solution for the analysis
and publication-grade graphical presentation of dithionite scramblase
assays, as well as a platform for review, dissemination and extension of the
strategies it employs. The package's name derives from a play on the fact
that lipid scrambling is also sometimes referred to as 'flipping'.
The package is originally published as Cotton, R.J., Ploier, B., Goren,
M.A., Menon, A.K., and Graumann, J. (2017). "flippant–An R package for the
automated analysis of fluorescence-based scramblase assays." BMC
Bioinformatics 18, 146. .