CA1d cells were plated on a 96-well plate and imaged on a BD Pathway 855 every 10 minutes for 92 hours on 11/22/2010. The image stack was analyzed manually and observed cell lifespans were recorded. Each cell lifespan is defined by a start event (mitosis) and an end event (mitosis, death or end of experiment). Start events are shared among siblings (sister cells) and assigned an identifier comprised of the frame number and a letter. End events are assigned an identifier of frame number and a letter if mitotic. In some cases a mitotic end event identifier corresponds to another start event identifier (i.e. cell progeny). Events are converted to time units based on the frame acquisition rates (sampling interval); cell birth time and lifespan are expressed in hours.
When a mitotic event occurs, the daughter cells are each assigned the next rows available in a data.frame.
The frame number of the event is recorded in the Birth.frame column of the rows assigned in (1).
The event from (1) is given an alphabetic identifier not previously used within the same Birth.frame.
The last frame a cell is observed in is recorded in the End.frame.
If the last frame is a mitotic event, assign letter as (3) in End.ID.
If the cell is present in the last frame of the image stack, the column End.of.Expt is marked TRUE, otherwise it is marked FALSE.
If a cell is observed to die, this is recorded in the column Death as a TRUE, otherwise as FALSE.
Create Birthtime column in hours by multiplying Birth.frame by 1/5.
Create Lifespan column in hours by multiplying (End.frame - Birth.frame) by 1/5.
Information about the cell line used (CA1d) can be found in the following reference: Fred R. Miller, Herbert D. Soule, Larty Tait, Robert J. Pauley, Sandra R. Wolman, Peter J. Dawson, and Gloria H. Heppner. Xenograft Model of Progressive Human Proliferative Breast Disease. JNCI J Natl Cancer Inst (1993) 85(21): 1725-1732 doi:10.1093/jnci/85.21.1725
data(ca1d.erlotinib)A data frame of lifespan CA1d observations in the presence of 16 micromolar erlotinib.
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data(ca1d.erlotinib)
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