ComputeAbsolute

Compute absolute expression in a grandR object and puts the normalized data into a new slot

Nucleotide conversion sequencing experiments have been
developed to add a temporal dimension to RNA-seq and single-cell RNA-seq. Such
experiments require specialized tools for primary processing such as GRAND-SLAM,
(see 'Jürges et al' <doi:10.1093/bioinformatics/bty256>) and specialized tools for
downstream analyses. 'grandR' provides a comprehensive toolbox for quality control,
kinetic modeling, differential gene expression analysis and visualization of such data.

Florian Erhard

grandR

Comprehensive Analysis of Nucleotide Conversion Sequencing Data

Teresa Rummel

Lygeri Sakellaridi

Kevin Berg

ComputeAbsolute function

<dl><dt>data</dt>
<dd>the grandR object</dd>
<dt>dilution</dt>
<dd>the dilution of the spikein transcript in the lysis reaction mix</dd>
<dt>volume</dt>
<dd>the approximate volume of the lysis chamber (nanoliters)</dd>
<dt>slot</dt>
<dd>the slot containing relative expression values</dd>
<dt>name</dt>
<dd>the name of the new slot to put absolute expression values in</dd></dl>

Arguments

Compute absolute expression using ERCC spike ins — ComputeAbsolute

<dl>

<dt>data</dt>
<dd>the grandR object</dd>


<dt>dilution</dt>
<dd>the dilution of the spikein transcript in the lysis reaction mix</dd>


<dt>volume</dt>
<dd>the approximate volume of the lysis chamber (nanoliters)</dd>


<dt>slot</dt>
<dd>the slot containing relative expression values</dd>


<dt>name</dt>
<dd>the name of the new slot to put absolute expression values in</dd>

</dl>

ComputeAbsolute: Compute absolute expression using ERCC spike ins

Description

Usage

Value

Arguments

See Also