NTRs can be computed from given new and total counts.
ComputePseudoNtr(
data,
new.slot,
total.slot = DefaultSlot(data),
detection.rate = 1
)a new grandR object
a grandR object
the slot containing new RNA counts
the slot containing total RNA counts
the detection rate of T-to-C mismatch reads (see details)
To correct for some bias, a detection rate (as suggested by Cao et al., Nature Biotech 2020) should be provided. This detection rate defines, how much new RNA is detected on average using the T-to-C mismatch reads.