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limma (version 3.22.7)

11.RNAseq: Topic: Analysis of RNA-seq Data

Description

This page gives an overview of the LIMMA functions available to analyze RNA-seq data.

voom
Transform RNA-seq or ChIP-seq counts to log counts per million (log-cpm) with associated precision weights. After this tranformation, RNA-seq or ChIP-seq data can be analyzed using the same functions as would be used for microarray data.

diffSplice
Test for differential splicing of exons between experimental conditions.

topSplice
Show a data.frame of top results from diffSplice.

plotSplice
Plot results from diffSplice.

Arguments

References

Law, CW, Chen, Y, Shi, W, Smyth, GK (2014). Voom: precision weights unlock linear model analysis tools for RNA-seq read counts. Genome Biology 15, R29. http://genomebiology.com/2014/15/2/R29