11.RNAseq: Topic: Analysis of RNA-seq Data
Description
This page gives an overview of the LIMMA functions available to analyze RNA-seq data.
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voom
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Transform RNA-seq or ChIP-seq counts to log counts per million (log-cpm) with associated precision weights.
After this tranformation, RNA-seq or ChIP-seq data can be analyzed using the same functions as would be used for microarray data.
diffSplice
Test for differential splicing of exons between experimental conditions.
topSplice
Show a data.frame of top results from diffSplice
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plotSplice
Plot results from diffSplice
.References
Law, CW, Chen, Y, Shi, W, Smyth, GK (2014).
Voom: precision weights unlock linear model analysis tools for RNA-seq read counts.
Genome Biology 15, R29.
http://genomebiology.com/2014/15/2/R29