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metacoder (version 0.3.7)

run_primersearch: Execute EMBOSS Primersearch

Description

Execute EMBOSS Primersearch

Usage

run_primersearch(
  seq_path,
  primer_path,
  mismatch = 5,
  output_path = tempfile(),
  program_path = "primersearch",
  ...
)

Value

The command generated as a character vector of length 1.

Arguments

seq_path

A character vector of length 1. The path to the fasta file containing reference sequences to search for primer matches in.

primer_path

A character vector of length 1. The path to the file containing primer pairs to match. The file should be whitespace-delimited with 3 columns: primer name, first primer sequence, and second primer sequence.

mismatch

An integer vector of length 1. The percentage of mismatches allowed.

output_path

A character vector of length 1. Where the output of primersearch is saved.

program_path

A character vector of length 1. The location of the primersearch binary. Ideally, it should be in your system's search path.

...

Additional arguments are passed to primersearch.

See Also

parse_primersearch