The 16S data come from Koren et al. (2011) and compared the bodysites oral, gut and plaque microbial communities in patients with atherosclerosis. The data can be analysed with our mixMC module. The data include 43 samples measured on 980 OTUs.
data(Koren.16S)A list containing two data sets, data.TSS and data.raw and some meta data information:
data.TSSdata frame with 43 rows (samples) and 980 columns (OTUs). The prefiltered normalised data using Total Sum Scaling normalisation.
data.rawdata frame with 43 rows (samples) and 980 columns (OTUs). The prefiltered raw count OTU data which include a 1 offset (i.e. no 0 values).
taxonomydata frame with 980 rows (OTUs) and 7 columns indicating the taxonomy of each OTU.
indivdata frame with 43 rows indicating sample meta data.
bodysitefactor of length 43 indicating the bodysite with levels arterial plaque, saliva and stool.
The data are from Koren et al. (2011) who examined the link between oral, gut and plaque microbial communities in patients with atherosclerosis and controls. Only healthy individuals were retained in the analysis. This study contained partially repeated measures from multiple sites including 15 unique patients samples from saliva and stool, and 13 unique patients only sampled from arterial plaque samples and we therefore considered a non multilevel analysis for that experimental design. After prefiltering, the data included 973 OTU for 43 samples. We strongly recommend to use log ratio transformations on the data.TSS normalisd data, as implemented in the PLS and PCA methods, see details on www.mixOmics.org/mixMC.
The data.raw include a 1 offset in order to be log ratios transformed after TSS normalisation. Consequently, the data.TSS are TSS normalisation of data.raw.
The CSS normalisation was performed on the orignal data (including zero values)
L<U+00EA> Cao K.-A., Costello ME, Lakis VA, Bartolo, F,Chua XY, Brazeilles R, Rondeau P. MixMC: Multivariate insights into Microbial Communities. PLoS ONE, 11(8): e0160169 (2016).
Koren, O., Spor, A., Felin, J., Fak, F., Stombaugh, J., Tremaroli, V., et al.: Human oral, gut, and plaque microbiota in patients with atherosclerosis. Proceedings of the National Academy of Sciences 108(Supplement 1), 4592-4598 (2011)