if(interactive()){
library("MotifDb")
matrix.fly <- query(MotifDb, "Dmelanogaster")
motifs <- as.list(matrix.fly)
motifs <- motifs[grepl("Dmelanogaster-FlyFactorSurvey-", names(motifs), fixed=TRUE)]
names(motifs) <- gsub("Dmelanogaster_FlyFactorSurvey_", "",
gsub("_FBgn[0-9]+$", "",
gsub("[^a-zA-Z0-9]","_",
gsub("(_[0-9]+)+$", "", names(motifs)))))
motifs <- motifs[unique(names(motifs))]
pfms <- sample(motifs, 50)
jaspar.scores <- MotIV::readDBScores(file.path(find.package("MotIV"),
"extdata", "jaspar2010_PCC_SWU.scores"))
d <- MotIV::motifDistances(lapply(pfms, pfm2pwm))
hc <- MotIV::motifHclust(d, method="average")
phylog <- hclust2phylog(hc)
leaves <- names(phylog$leaves)
pfms <- pfms[leaves]
pfms <- lapply(names(pfms), function(.ele, pfms){new("pfm",mat=pfms[[.ele]],
name=.ele)},pfms)
pfms <- DNAmotifAlignment(pfms, minimalConsensus=3)
plotMotifStackWithPhylog(phylog, pfms, f.phylog=0.3,
cleaves = 0.5, clabel.leaves = 0.7)
}
Run the code above in your browser using DataLab