Detects spots for one file
find.spots.file(
file,
dir,
color,
thresh = NULL,
thresh.auto = FALSE,
thresh.quantile = 0.9,
filter = NULL,
cores = 1
)file
directory for results
which color, images have to be in folder with color name
threshold
Logical. Find threshold automatically?
numeric. use simple
2d-filter to use before spot detection
number of cores to use in parallel (with parallel package only)
spot images in spot-color/, number of spots as txt files in spot-color/