Optical absorption spectra of chlorophyll \(a\) in methanol and chlorophylls \(a\) and \(b\) in diethyl ether containing the wavelengths at 1 nm interval.
A filter_mspct with three member filter_spct objects
each with variable number of rows and 2 numeric variables, w.length
and A
The variables of the member spectra are as follows:
w.length (nm)
A (spectral absorbance)
Data from PhotochemCAD 2.1a has been munged on 2 June 2017 by Scott Prahl (https://omlc.org/) to make the information available to non-Windows users. Although he has tried to be as careful as possible, he may have introduced some error; the cautious user is advised to compare these results with the original sources at https://www.photochemcad.com/ (Du et al., 1998; Dixon et al., 2005).
Fluorescence emission was measured using a Spex FluoroMax. The excitation and emission monochromators were set at 1 mm, giving a spectral bandwidth of 4.25 nm. The data interval was 0.5 nm and the integration time was 2.0 sec. Samples were prepared in 1cm path length quartz cells with absorbance less than 0.1 at the excitation and all emission wavelengths to uniformly illuminate across the sample, and to avoid the inner-filter effect. The dark counts were subtracted and the spectra were corrected for wavelength-dependent instrument sensitivity.
J. M. Dixon, M. Taniguchi and J. S. Lindsey "PhotochemCAD 2. A refined program with accompanying spectral databases for photochemical calculations", Photochem. Photobiol., 81, 212-213, 2005.
H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design and research tool in photochemistry," Photochem. Photobiol., 68, 141-142, 1998.
names(chlorophylls_fluorescence.mspct)
getWhatMeasured(chlorophylls_fluorescence.mspct[[1]])
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