The purpose of msms
is for the demonstration of the peptide
identification methods, e.g., peakplot
or psm
.
msms
contains two tandem mass spectra meassured on a linear ion
trap coupled to an Orbitrap mass analyzer
(Thermo Scientific, Bremen, Germany).
The protein sample originates from 10
mouse brain homogenate, prepared in sterile PBS containing protease
inhibitors (Complete; Riche, Switzerland) by repeated extrusion through
syringe needles of successive smaller size. To remove gross cellular
debris the homogenate was centrifuged for 10 min at 2400 rpm
at 4 degrees centigrade.
The data were acquired withing the Sixth Framework Programm (FP6-37457).
The project was called SysProt.