qpcR (version 1.3-7.1)

S27: qPCR data of the S27a transcript in 15 testicular biopsies

Description

The data was obtained from cDNA reverse transcribed from the isolated RNA of 15 different testicular biopsies.

Usage

S27

format

A data frame with cycle number and 15 different qPCR runs (F1 - F15).

source

Andrej-Nikolai Spiess & Caroline Feig, Department of Andrology, University Hospital Hamburg, Germany.

Details

The real-time PCR was conducted with primers for the S27a housekeeping gene in a Lightcycler 1.0 instrument (Roche Diagnostics).

Examples

m1 <- pcrfit(S27, 1, 2, l5)
plot(m1)