qpcR (version 1.3-7.1)

midpoint: Calculation of the 'midpoint' region

Description

Calculates the exponential region midpoint using the algorithm described in Peirson et al. (2003).

Usage

midpoint(object, noise.cyc = 1:5)

Arguments

object

a fitted object of class 'pcrfit'.

noise.cyc

the cycles defining the background noise.

Value

• A list with the following components:
• f.mp
  the 'midpoint' fluorescence.
• cyc.mp
  the 'midpoint' cycle, as predicted from f.mp.

Details

The 'midpoint' region is calculated by $$F_{noise} \cdot \sqrt{\frac{F_{max}}{F_{noise}}}$$ with $F_{noise}$ = the standard deviation of the background cycles and $F_{max}$ = the maximal fluorescence.

References

Experimental validation of novel and conventional approaches to quantitative real-time PCR data analysis. Peirson SN, Butler JN & Foster RG. Nucleic Acids Research (2003), 31: e73.

Examples

m1 <- pcrfit(reps, 1, 2, l5)
mp <- midpoint(m1) 
plot(m1)
abline(h = mp$f.mp, col = 2)
abline(v = mp$mp, col = 2)