min_flow: Minimum flow rate to support MO2

Description

Calculates the minimum flow rate into a respirometer required to maintain a high pO2. Useful for ensuring an acclimating animal maintains a normoxic environment. It can also be used to estimate the flow rate needed for a given pO2 decrease desired for flow-through respirometry.

Usage

min_flow(
  MO2,
  min_pO2 = 90,
  pO2_in = 100,
  temp = 25,
  sal = 35,
  atm_pres = 1013.25
)

Arguments

MO2

whole-animal oxygen consumption rate (umol / hour).

min_pO2

minimum pO2 acceptable in respirometer (% air saturation). Default is 90% air saturation.

pO2_in

pO2 of water entering respirometer (% air saturation). Default is 100% air saturation.

temp

temperature (<U+00B0>C). Default is 25 <U+00B0>C.

sal

salinity (psu). Default is 35 psu.

atm_pres

atmospheric pressure (mbar). Default is 1013.25 mbar.

Value

The flow rate (liters / min) into the respirometer required for the steady state pO2 to be min_pO2.

References

Steffensen JF. 1989. Some errors in respirometry of aquatic breathers: How to avoid and correct for them. Fish Physiol Biochem. 6:49<U+2013>59. Equation 8.

Examples

Run this code

# NOT RUN {
min_flow(MO2 = 1000)

# What is the minimum flow rate required to maintain at least 75% air saturation in a
# respirometer with an organism(s) with an oxygen consumption rate of 1000 umol/h
# when the intake fresh water is 10 <U+00B0>C and 90% air saturated?
min_flow(MO2 = 1000, min_pO2 = 75, pO2_in = 90, temp = 10, sal = 0)

# }