respirometry (version 2.0.0)

co2_rate: Calculate CO2 to add to a respirometer intake flow

Description

Calculates the moles of CO2 gas to be added to a respirometer intake seawater flow to achieve the desired pCO2 in the respirometer. Useful for ocean acidification experiments where CO2 treatments are desired. Can be used for acclimation before a trial begins or for use with flow-through respirometry.

Usage

co2_rate(
  goal_pco2,
  init_pH,
  flow_rate,
  temp = 25,
  sal = 35,
  TA = NULL,
  atm_pres = 1013.25,
  MO2 = NULL,
  RQ = 1
)

Value

moles of CO2 gas to be added to the intake flow per minute.

Arguments

goal_pco2

the desired pCO2 in the respirometer (uatm).

init_pH

ambient pH of the intake flow (total scale).

flow_rate

rate of water flow into the respirometer (liters / minute).

temp

temperature (°C). Default is 25 °C.

sal

salinity (psu). Default is 35 psu. If sal < 26 psu, then TA must be provided.

TA

(optional) total alkalinity (umol / kg). If undefined TA is estimated from salinity using guess_TA.

atm_pres

atmospheric pressure (mbar). Default is 1013.25 mbar.

MO2

(optional) oxygen consumption rate (umol / hr). If defined, the CO2 to be added is reduced to compensate for the CO2 produced by the organism.

RQ

(optional) respiratory quotient: ratio of CO2 produced / O2 consumed. Only used if MO2 is defined. Default is 1.

Author

Matthew A. Birk,

References

Jokiel PL, Bahr KD, Rodgers KS. 2014. Low-cost, high-flow mesocosm system for simulating ocean acidification with CO2 gas. Limnol Oceanogr Methods. 12:313–322.

See Also

co2_add, flush_carb, carb, peri_pump

Examples

# I want the respirometer to have a pCO2 = 1000 uatm. How much CO2 per minute do I need
# to add to the intake flow if the ambient pH is 8.1 and it is flowing at 3 LPM?
co2_rate(goal_pco2 = 1000, init_pH = 8.1, flow_rate = 3)