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spp (version 1.16.0)

read.eland.tags: Read eland output file

Description

Reads in ELAND output file, returning 5'-end tag coordinates and number of mismatches associated with each mapped tag.

Usage

read.eland.tags(filename, 
  read.tag.names = F, 
  fix.chromosome.names = T, 
  max.eland.tag.length = -1,
  extended=F, 
  multi = F)

Arguments

filename

ELAND output file

read.tag.names

Whether the tag names should be read in

fix.chromosome.names

Whether to remove ".fa" from the end of the sequence names

max.eland.tag.length

Specifies max length of the tag sequence considered by ELAND. This needs to be specified if the tags are longer than the sequences considred by ELAND during alignment.

extended

Whether the file is written out in "extended" format provided in GA pipeline 1.0.

multi

Whether the file is written in "multi" format, showing multiple alignments of the reads

Value

tags

A vector of 5' tag coordinates, with negative values corresponding to tags mapped to the negative strand.

quality

Number of mismatches

names

Tag names, if read.tag.names was set